Diagnostic Protocol for Monilinia fructigena (Apple Brown Rot)

1 Introduction

1.1 General Introduction
1.2 Host Range
1.3 Geographic Distribution
1.4 Disease Cycle
1.5 Symptoms

2 Taxonomic Information

3 Detection

3.1 Fruit, Blossom and Stem Cankers
3.2 Sampling, Isolation and Detection Methods

4 Identification

4.1 Morphological and Cultural Methods
4.2 Polymerase Chain Reaction (PCR) from Pure Monilinia Culture

5 Records

5.1 Specimen Submission Form (Sample Form)
5.2 Laboratory Work Form (Sample Form)
5.3 DNA Extraction Work Form (Sample Form)
5.4 PCR Record Form (Sample Form)
5.5 Synoptic Key Record Work Form (Sample Form)
5.6 Gel Electrophoresis Work Form (Sample Form)

6 Contact Points

7 Acknowledgements

8 References

Appendix 1: DNA Extraction from Fungal Mycelium using Webster Method

Appendix 2: PCR primers for M. fructicola and M. laxa

Prepared for the Subcommittee on Plant Health Diagnostic Standards (SPHDS)

In accordance with SPHDS RS 2